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Gamma Catenin Monoclonal antibody, PBS Only

Gamma Catenin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, Canine, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5D10

Cat no : 66445-1-PBS

Print datasheet

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

验证数据展示

  • WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

    WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

    IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JUP antibody included
  • IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

    IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JUP antibody included
  • IF Staining of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

    IF Staining of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66445-1-Ig (same clone as 66445-1-PBS)

    IF Staining of MCF-7 using 66445-1-Ig (same clone as 66445-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.

产品信息

66445-1-PBS targets Gamma Catenin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, Canine, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat, pig, Canine, mouse
Immunogen Gamma Catenin fusion protein Ag1627 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name junction plakoglobin
Synonyms ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC011865
Gene Symbol Gamma Catenin
Gene ID (NCBI) 3728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14923
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.