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验证数据展示

  • WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

    WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

    U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

    WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Gelsolin Monoclonal antibody, PBS Only

Gelsolin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66280-1-PBS

产品说明书

CloneNo. 3C4A2

宿主/亚型

Mouse / IgA

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

66280-1-PBS targets Gelsolin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Gelsolin fusion protein Ag17916 种属同源性预测
宿主/亚型 Mouse / IgA
抗体类别 Monoclonal
产品类型 Antibody
全称 gelsolin (amyloidosis, Finnish type)
别名 Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN
计算分子量 782 aa, 86 kDa
观测分子量 80-83 kDa
GenBank蛋白编号BC026033
基因名称 Gelsolin
Gene ID (NCBI) 2934
RRIDAB_2881663
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP06396
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.