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验证数据展示

  • WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

    WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

    U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

    WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Gelsolin Monoclonal antibody, PBS Only

Gelsolin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66280-1-PBS
CloneNo. 3C4A2

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

66280-1-PBS targets Gelsolin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Gelsolin fusion protein Ag17916 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name gelsolin (amyloidosis, Finnish type)
Synonyms Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN
Calculated Molecular Weight 782 aa, 86 kDa
Observed Molecular Weight 80-83 kDa
GenBank Accession NumberBC026033
Gene Symbol Gelsolin
Gene ID (NCBI) 2934
RRIDAB_2881663
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP06396
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.