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GlyT2 Polyclonal antibody

GlyT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25948-1-AP

Print datasheet

Synonyms

SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2

验证数据展示

  • WB analysis using 25948-1-AP

    WB analysis using 25948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25948-1-AP (GlyT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 25948-1-AP

    IHC staining of human skin cancer using 25948-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25948-1-AP (GlyT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 25948-1-AP

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 25948-1-AP

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25948-1-AP targets GlyT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GlyT2 fusion protein Ag22835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2
Calculated Molecular Weight 797 aa, 87 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC096319
Gene Symbol GlyT2
Gene ID (NCBI) 9152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y345
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GlyT2 is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. Like other members of the family, GlyT2 is trafficked to the cell surface as homo-oligomers from the endoplasmic reticulum (ER) (PMID: 32714574). GlyT2 is expressed in medulla, and to a lesser extent in spinal cord and cerebellum. GlyT2 has 3 isoforms with the molecular mass of 63, 86 and 87 kDa. The 63 kDa band is the unglycosylated GlyT2 polypeptide, whereas the 70-110 kDa band is a differential glycosylation form of GlyT2 (PMID: 17851090, 17962467).

实验方案

Product Specific Protocols
WB protocol for GlyT2 antibody 25948-1-APDownload protocol
IHC protocol for GlyT2 antibody 25948-1-APDownload protocol
IF protocol for GlyT2 antibody 25948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols