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验证数据展示

  • WB analysis using 25948-1-AP

    WB analysis using 25948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25948-1-AP (GlyT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 25948-1-AP

    IHC staining of human skin cancer using 25948-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25948-1-AP (GlyT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 25948-1-AP

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 25948-1-AP

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GlyT2 Polyclonal antibody

GlyT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 25948-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25948-1-AP targets GlyT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GlyT2 fusion protein Ag22835 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, glycine), member 5
别名 SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2
计算分子量 797 aa, 87 kDa
观测分子量70 kDa
GenBank蛋白编号BC096319
基因名称 GlyT2
Gene ID (NCBI) 9152
RRIDAB_3085831
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y345
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GlyT2 is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. Like other members of the family, GlyT2 is trafficked to the cell surface as homo-oligomers from the endoplasmic reticulum (ER) (PMID: 32714574). GlyT2 is expressed in medulla, and to a lesser extent in spinal cord and cerebellum. GlyT2 has 3 isoforms with the molecular mass of 63, 86 and 87 kDa. The 63 kDa band is the unglycosylated GlyT2 polypeptide, whereas the 70-110 kDa band is a differential glycosylation form of GlyT2 (PMID: 17851090, 17962467).

实验方案

Product Specific Protocols
WB protocol for GlyT2 antibody 25948-1-APDownload protocol
IHC protocol for GlyT2 antibody 25948-1-APDownload protocol
IF protocol for GlyT2 antibody 25948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols