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验证数据展示

  • WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

    WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50422-2

    Cytometric bead array standard curve of MP50422-2

    Cytometric bead array standard curve of MP50422-2, GYPA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66778-2-PBS. Detection antibody: 66778-1-PBS. Standard:Ag8635. Range: 0.391-100 ng/mL.

Glycophorin A/CD235a Monoclonal antibody, PBS Only (Detector)

Glycophorin A/CD235a Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66778-1-PBS

产品说明书

CloneNo. 2B12G10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

Glycophorin A, GPErik, GPA, Glycophorin-A, CD235a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

产品信息

66778-1-PBS targets Glycophorin A/CD235a as part of a matched antibody pair:

MP50422-2: 66778-2-PBS capture and 66778-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Glycophorin A/CD235a fusion protein Ag8635 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 Glycophorin A, GPErik, GPA, Glycophorin-A, CD235a
计算分子量 150 aa, 16 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_2882124
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02724
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)