• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Glycophorin A Monoclonal antibody, PBS Only

Glycophorin A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12G10

Cat No : 66778-1-PBS

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

    WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

产品信息

66778-1-PBS targets Glycophorin A in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Glycophorin A fusion protein Ag8635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC005319
Gene Symbol GYPA
Gene ID (NCBI) 2993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02724
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)