• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Progranulin/PGRN Polyclonal antibody

Progranulin/PGRN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29491-1-AP

Print datasheet

Synonyms

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

验证数据展示

  • WB analysis using 29491-1-AP

    WB analysis using 29491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29491-1-AP

    WB analysis using 29491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, HEK-293 cells, HepG2 cells, MCF-7 cells, U-937 cells, J774A.1 cells, NIH/3T3 cells, RAW 264.7 cells, mouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy GRN Ready-To-Use IHC Kit. GRN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29491-1-AP targets Progranulin/PGRN in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Progranulin/PGRN fusion protein Ag30723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name granulin
Synonyms granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88
Calculated Molecular Weight 64 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC000324
Gene Symbol Granulin
Gene ID (NCBI) 2896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28799
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

实验方案

Product Specific Protocols
WB protocol for Progranulin/PGRN antibody 29491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols