GSDMD Recombinant antibody

GSDMD Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83562-3-RR
CloneNo. 240548B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, EL-4 cells, RAW 264.7 cells, J774A.1 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83562-3-RR targets GSDMD in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen GSDMD fusion protein Ag34905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin D
Synonyms Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049
Calculated Molecular Weight53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC029813
Gene Symbol Gsdmd
Gene ID (NCBI) 69146
RRIDAB_3671177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9D8T2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 83562-3-RRDownload protocol
IHC protocol for GSDMD antibody 83562-3-RRDownload protocol
IF protocol for GSDMD antibody 83562-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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