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GSDMD Recombinant antibody

GSDMD Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240548B10

Cat no : 83562-3-RR

Print datasheet

Synonyms

Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049

验证数据展示

  • WB analysis of NIH/3T3 using 83562-3-RR

    WB analysis of NIH/3T3 using 83562-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of RAW 264.7 using 83562-3-RR

    FC experiment of RAW 264.7 using 83562-3-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Gsdmd Recombinant antibody (83562-3-RR, Clone:240548B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83562-3-RR

    Affinity and Kinetic Characterization of 83562-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83562-3-RR against Mouse Gsdmd were performed. The affinity constant is 1.19 nM.

经过测试的应用

Positive WB detected inNIH/3T3 cells, EL-4 cells, RAW 264.7 cells, J774A.1 cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83562-3-RR is GSDMD Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen GSDMD fusion protein Ag34905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin D
Synonyms Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049
Calculated Molecular Weight53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC029813
Gene Symbol Gsdmd
Gene ID (NCBI) 69146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9D8T2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 83562-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols