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验证数据展示

  • WB analysis of NIH/3T3 using 83562-3-RR

    WB analysis of NIH/3T3 using 83562-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83562-3-RR

    IHC staining of mouse spleen using 83562-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83562-3-RR (GSDMD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83562-3-RR

    IHC staining of mouse spleen using 83562-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83562-3-RR (GSDMD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 83562-3-RR

    IF Staining of NIH/3T3 using 83562-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Gsdmd antibody (83562-3-RR, Clone: 240548B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of RAW 264.7 using 83562-3-RR

    FC experiment of RAW 264.7 using 83562-3-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Gsdmd Recombinant antibody (83562-3-RR, Clone:240548B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83562-3-RR

    Affinity and Kinetic Characterization of 83562-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83562-3-RR against Mouse Gsdmd were performed. The affinity constant is 1.19 nM.

GSDMD Recombinant antibody

GSDMD Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83562-3-RR
CloneNo. 240548B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, EL-4 cells, RAW 264.7 cells, J774A.1 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83562-3-RR targets GSDMD in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen GSDMD fusion protein Ag34905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin D
Synonyms Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049
Calculated Molecular Weight53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC029813
Gene Symbol Gsdmd
Gene ID (NCBI) 69146
RRIDAB_3671177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9D8T2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 83562-3-RRDownload protocol
IHC protocol for GSDMD antibody 83562-3-RRDownload protocol
IF protocol for GSDMD antibody 83562-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols