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验证数据展示

  • WB analysis using 26875-1-AP

    WB analysis using 26875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26875-1-AP

    IF Staining of HepG2 using 26875-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A549 using 26875-1-AP

    WB analysis of A549 using 26875-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 26875-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26875-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26875-1-AP (H2AFY antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

H2AFY Polyclonal antibody

H2AFY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26875-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

H2AF12M, H2A/y, H2A.y, H2A histone family, member Y, Core histone macro-H2A.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Caco-2 cells, mouse brain tissue, HeLa cells, rat brain
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26875-1-AP targets H2AFY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 H2AFY fusion protein Ag25445 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 H2A histone family, member Y
别名 H2AF12M, H2A/y, H2A.y, H2A histone family, member Y, Core histone macro-H2A.1
计算分子量 372 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC013331
基因名称 H2AFY
Gene ID (NCBI) 9555
RRIDAB_2918113
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75367
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for H2AFY antibody 26875-1-APDownload protocol
IHC protocol for H2AFY antibody 26875-1-APDownload protocol
IF protocol for H2AFY antibody 26875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IP

Cell Death Discov

CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation

Authors - Ting Zhao
View Article