H3F3B Polyclonal antibody
H3F3B Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
10265-1-AP targets H3F3B in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | H3F3B fusion protein Ag0326 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | H3 histone, family 3B (H3.3B) |
Synonyms | H3F3A, H3F3, H3-3B, H3-3A, H3.3B |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC001124 |
Gene Symbol | H3F3B |
Gene ID (NCBI) | 3021 |
RRID | AB_513398 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P84243 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].
实验方案
Product Specific Protocols | |
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WB protocol for H3F3B antibody 10265-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Commun Signal Reciprocal regulation between RACGAP1 and AR contributes to endocrine therapy resistance in prostate cancer | ||
Immunobiology Exploring liquid-liquid phase separation-related diagnostic biomarkers in osteoarthritis based on machine learning algorithms and experiment |