ChromoTek HA VHH, recombinant binding protein
Alpaca anti-HA-Tag VHH, purified recombinant binding protein
Specificity
HA-Tag (sequence YPYDVPDYA)
Applications
Conjugation
Type
Nanobody
Conjugate
Unconjugated
Cat No : at
Synonyms
Validation Data Gallery
Product Information
Alpaca anti-HA-Tag VHH, purified recombinant binding protein
| Applications | Suitable for conjugation to beads, plates, and surfaces via NHS-chemistry |
| Specificity/Target | Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low. |
| Conjugate | Unconjugated |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions |
| Molecular Weight | 14.1 kDa |
| RRID | AB_3096297 |
| Storage Buffer | 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
Documentation
| SDS |
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| HA VHH, recombinant binding protein SDS (PDF) |
| Protocol |
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| HA VHH, recombinant binding protein (PDF) |