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  • Featured Product
  • KD/KO Validated

HADHA Monoclonal antibody, PBS Only

HADHA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B1

Cat No : 60250-1-PBS

Print datasheet

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

验证数据展示

  • IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHA antibody included
  • IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHA antibody included
Planning an IHC experiment? We recommend our IHCeasy HADHA Ready-To-Use IHC Kit. HADHA primary antibody included.

产品信息

60250-1-PBS targets HADHA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen HADHA fusion protein Ag1211 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Synonyms 78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC009235
Gene Symbol HADHA
Gene ID (NCBI) 3030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40939
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).