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验证数据展示

  • WB analysis of human placenta using 27593-1-AP

    WB analysis of human placenta using 27593-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27593-1-AP (HAI-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27593-1-AP

    IHC staining of human lung cancer using 27593-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27593-1-AP (HAI-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27593-1-AP

    IHC staining of human lung cancer using 27593-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27593-1-AP (HAI-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brown disease using 27593-1-AP

    IHC staining of human brown disease using 27593-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27593-1-AP (HAI-1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HAI-1 Polyclonal antibody

HAI-1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27593-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SPINT1, HAI 1, HAI, MANSC2, Kunitz-type protease inhibitor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27593-1-AP targets HAI-1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 HAI-1 fusion protein Ag26733 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type 1
别名 SPINT1, HAI 1, HAI, MANSC2, Kunitz-type protease inhibitor 1
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC004140
基因名称 HAI-1
Gene ID (NCBI) 6692
RRIDAB_2880918
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAI-1 antibody 27593-1-APDownload protocol
IHC protocol for HAI-1 antibody 27593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols