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HAL Polyclonal antibody

HAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25940-1-AP

Print datasheet

Synonyms

HAL, HIS, Histidase, histidine ammonia lyase, HSTD

验证数据展示

  • WB analysis using 25940-1-AP

    WB analysis using 25940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25940-1-AP (HAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 25940-1-AP

    IHC staining of human skin using 25940-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25940-1-AP (HAL Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HAL antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman skin tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HAL Ready-To-Use IHC Kit. HAL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 25940-1-AP is HAL Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen HAL fusion protein Ag22985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histidine ammonia-lyase
Synonyms HAL, HIS, Histidase, histidine ammonia lyase, HSTD
Calculated Molecular Weight 657 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC096097
Gene Symbol HAL
Gene ID (NCBI) 3034
RRIDAB_2880303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42357
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa.

实验方案

Product Specific Protocols
WB protocol for HAL antibody 25940-1-APDownload protocol
IHC protocol for HAL antibody 25940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell Death Dis

Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway

Authors - Guoqing Xie
View Article
WB

Autophagy

The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple

Authors - Runmin Che
View Article
mouseWB

FASEB J

Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment

Authors - Kenneth N Maclean
View Article