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验证数据展示

  • WB analysis of mouse liver using 25056-1-AP

    WB analysis of mouse liver using 25056-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HAO1 Polyclonal antibody

HAO1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25056-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25056-1-AP targets HAO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HAO1 fusion protein Ag21884 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxyacid oxidase (glycolate oxidase) 1
别名 Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1
计算分子量 370 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC113665
基因名称 HAO1
Gene ID (NCBI) 54363
RRIDAB_2879875
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAO1 antibody 25056-1-APDownload protocol
IHC protocol for HAO1 antibody 25056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols