验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells, mouse kidney tissue, HepG2 cells, rat kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
16563-1-AP targets HAO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | HAO2 fusion protein Ag9838 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxyacid oxidase 2 (long chain) |
| 别名 | (S)-2-hydroxy-acid oxidase, peroxisomal, 2-Hydroxyacid oxidase 2, Cell growth-inhibiting gene 16 protein, EC:1.1.3.15, GIG16 |
| 计算分子量 | 351 aa, 39 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC020863 |
| 基因名称 | HAO2 |
| Gene ID (NCBI) | 51179 |
| RRID | AB_3669250 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYQ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HAO2 antibody 16563-1-AP | Download protocol |
| IF protocol for HAO2 antibody 16563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
