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验证数据展示

  • WB analysis of fetal human brain using 25133-1-AP

    WB analysis of fetal human brain using 25133-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25133-1-AP

    IHC staining of mouse brain using 25133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HAP1 antibody included
  • IHC staining of mouse brain using 25133-1-AP

    IHC staining of mouse brain using 25133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HAP1 antibody included
  • IF Staining of SH-SY5Y using 25133-1-AP

    IF Staining of SH-SY5Y using 25133-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 25133-1-AP (HAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HAP1 Polyclonal antibody

HAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25133-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HAP 1, HAP1, HAP2, hHLP1, HIP5, HLP, HLP1, Neuroan 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HAP1 Ready-To-Use IHC Kit. HAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25133-1-AP targets HAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 HAP1 fusion protein Ag17705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin-associated protein 1
别名 HAP 1, HAP1, HAP2, hHLP1, HIP5, HLP, HLP1, Neuroan 1
计算分子量 671 aa, 76 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC156728
基因名称 HAP1
Gene ID (NCBI) 9001
RRIDAB_2879915
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54257
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).

实验方案

Product Specific Protocols
WB protocol for HAP1 antibody 25133-1-APDownload protocol
IHC protocol for HAP1 antibody 25133-1-APDownload protocol
IF protocol for HAP1 antibody 25133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FEBS Open Bio

Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins

Authors - Kazuma Yoshioka
View Article
humanIHC,WB

Mol Biol Rep

Huntingtin-associated protein 1 is a potential tumor suppressor for gastric cancer

Authors - Ye-Min Qu
View Article
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article