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验证数据展示

  • IHC staining of mouse cerebellum using 26831-1-AP

    IHC staining of mouse cerebellum using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 26831-1-AP

    IHC staining of mouse cerebellum using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 26831-1-AP

    IHC staining of mouse placenta using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 26831-1-AP

    IHC staining of mouse placenta using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 26831-1-AP

    IF Staining of U2OS using 26831-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HAPLN1 antibody (26831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

HAPLN1 Polyclonal antibody

HAPLN1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 26831-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Cartilage link protein, Cartilage linking protein 1, CRTL1, HAPLN1, Proteoglycan link protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse placenta tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26831-1-AP targets HAPLN1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 HAPLN1 fusion protein Ag25221 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyaluronan and proteoglycan link protein 1
别名 Cartilage link protein, Cartilage linking protein 1, CRTL1, HAPLN1, Proteoglycan link protein
计算分子量 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC057808
基因名称 HAPLN1
Gene ID (NCBI) 1404
RRIDAB_3669570
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10915
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453).

实验方案

Product Specific Protocols
IHC protocol for HAPLN1 antibody 26831-1-APDownload protocol
IF protocol for HAPLN1 antibody 26831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols