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HAPLN4 Polyclonal antibody

HAPLN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21228-1-AP

Print datasheet

Synonyms

Brain link protein 2, BRAL2, HAPLN4, KIAA1926

验证数据展示

  • WB analysis of mouse brain using 21228-1-AP

    WB analysis of mouse brain using 21228-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21228-1-AP (HAPLN4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21228-1-AP

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 21228-1-AP

    IHC staining of human brain using 21228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21228-1-AP (HAPLN4 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21228-1-AP is HAPLN4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HAPLN4 fusion protein Ag15687 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hyaluronan and proteoglycan link protein 4
Synonyms Brain link protein 2, BRAL2, HAPLN4, KIAA1926
Calculated Molecular Weight 402 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC142644
Gene Symbol HAPLN4
Gene ID (NCBI) 404037
RRIDAB_10695504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAPLN4 antibody 21228-1-APDownload protocol
IHC protocol for HAPLN4 antibody 21228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Diabetes

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Authors - Yuguo Niu
View Article