验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HCT 116 cells, HT-29 cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83204-2-RR targets HAS2 in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | HAS2 fusion protein Ag34945 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | hyaluronan synthase 2 |
Synonyms | Hyaluronic acid synthase 2, Hyaluronate synthase 2, hyaluronan synthase 2, HA synthase 2, EC:2.4.1.212 |
Calculated Molecular Weight | 552 aa, 64 kDa |
Observed Molecular Weight | 63-67 kDa |
GenBank Accession Number | BC069353 |
Gene Symbol | HAS2 |
Gene ID (NCBI) | 3037 |
RRID | AB_3670892 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92819 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.
实验方案
Product Specific Protocols | |
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WB protocol for HAS2 antibody 83204-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |