TIM3 Polyclonal antibody

TIM3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11872-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

T-cell membrane protein 3, T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, Daudi cells, Raji cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11872-1-AP targets TIM3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 TIM3 fusion protein Ag2459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell membrane protein 3, T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2
计算分子量 301 aa, 33 kDa
观测分子量 50-70 kDa, 33 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
RRIDAB_10734330
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)

实验方案

Product Specific Protocols
WB protocol for TIM3 antibody 11872-1-APDownload protocol
IHC protocol for TIM3 antibody 11872-1-APDownload protocol
IF protocol for TIM3 antibody 11872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease

Authors - Qinyu Peng
humanWB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li
humanIHC

CNS Neurosci Ther

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

Authors - Hao Zhang

Int J Oncol

Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.

Authors - Jiaxing Yang
mouseWB

Iran J Immunol

The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis.

Authors - Min Lin
humanWB

Immun Inflamm Dis

Galectin-9 and myeloid-derived suppressor cell as prognostic indicators for chronic lymphocytic leukemia

Authors - Xierenguli Alimu
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