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HAX1 Polyclonal antibody

HAX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11266-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (3)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

HS1-binding protein 1, HS1-associating protein X-1, HCLSBP1, HCLS1-associated protein X-1, HAX-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11266-1-AP targets HAX1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, pig, monkey, hamster
免疫原 HAX1 fusion protein Ag1792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HCLS1 associated protein X-1
别名 HS1-binding protein 1, HS1-associating protein X-1, HCLSBP1, HCLS1-associated protein X-1, HAX-1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC014314
基因名称 HAX1
Gene ID (NCBI) 10456
RRIDAB_2263720
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.

实验方案

Product Specific Protocols
WB protocol for HAX1 antibody 11266-1-APDownload protocol
IHC protocol for HAX1 antibody 11266-1-APDownload protocol
IF protocol for HAX1 antibody 11266-1-APDownload protocol
IP protocol for HAX1 antibody 11266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Authors - Michael Heider
hamsterWB,IP

Nat Commun

Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1.

Authors - Yalan Zhang
humanWB

J Cell Biol

Hax1 regulates neutrophil adhesion and motility through RhoA.

Authors - Cavnar Peter J PJ
  • KD Validated
mouseWB

FASEB J

Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice.

Authors - Yalan Zhang
humanWB, IHC

J Cell Mol Med

HAX1 maintains the glioma progression in hypoxia through promoting mitochondrial fission.

Authors - Jinghui Lin
humanWB

Fish Shellfish Immunol

Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress.

Authors - Ying Shi
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