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验证数据展示

  • WB analysis using 67883-2-Ig

    WB analysis using 67883-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67883-2-Ig

    IF Staining of HeLa using 67883-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HEK-293 using 67883-2-Ig

    WB analysis of HEK-293 using 67883-2-Ig

    WB result of HAX1 antibody (67883-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

HAX1 Monoclonal antibody

HAX1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67883-2-Ig

产品说明书

CloneNo. 2A12F11
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

FLJ17042, FLJ18492, FLJ93803, HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

CoIPSee 1 publications below

产品信息

67883-2-Ig targets HAX1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用CoIP
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 HAX1 fusion protein Ag27244 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 HCLS1 associated protein X-1
别名 FLJ17042, FLJ18492, FLJ93803, HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3
计算分子量 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC014314
基因名称 HAX1
Gene ID (NCBI) 10456
RRIDAB_2918640
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAX1 antibody 67883-2-IgDownload protocol
IF protocol for HAX1 antibody 67883-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Phytomedicine

Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts

Authors - Tingting Zhang
View Article