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验证数据展示

  • WB analysis of human placenta using 19990-1-AP

    WB analysis of human placenta using 19990-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 19990-1-AP

    IHC staining of human colon using 19990-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 19990-1-AP (Hemoglobin Alpha-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Hemoglobin Alpha-Specific Polyclonal antibody

Hemoglobin Alpha-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19990-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HBA1, Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, human heart tissue, human spleen tissue
Positive IHC detected inhuman colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19990-1-AP targets Hemoglobin Alpha-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, alpha 1
别名 HBA1, Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin
计算分子量 16 kDa
观测分子量 14-16 kDa
GenBank蛋白编号NM_000558
基因名称 HBA1
Gene ID (NCBI) 3039
RRIDAB_11182500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP69905
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.

实验方案

Product Specific Protocols
WB protocol for Hemoglobin Alpha-Specific antibody 19990-1-APDownload protocol
IHC protocol for Hemoglobin Alpha-Specific antibody 19990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols