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验证数据展示

  • WB analysis of human plasma using 16824-1-AP

    WB analysis of human plasma using 16824-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16824-1-AP

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16824-1-AP

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 40x lens).

HBD Polyclonal antibody

HBD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16824-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman blood tissue, human plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16824-1-AP targets HBD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 HBD fusion protein Ag10409 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, delta
别名 Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta
计算分子量 147 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC070282
基因名称 HBD
Gene ID (NCBI) 3045
RRIDAB_2114476
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HBD antibody 16824-1-APDownload protocol
IHC protocol for HBD antibody 16824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article
humanWB

Placenta

SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

Authors - Yuxuan Jiang
View Article