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验证数据展示

  • WB analysis using 25728-1-AP

    WB analysis using 25728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25728-1-AP (HBG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 25728-1-AP

    IP experiment of K-562 using 25728-1-AP

    IP result of anti-HBG1 (IP:25728-1-AP, 3ug; Detection:25728-1-AP 1:300) with K-562 cells lysate 2000ug.

HBG1 Polyclonal antibody

HBG1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25728-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA and More (1)

别名

Gamma 1 globin, Hb F Agamma, HBG1, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, human placenta tissue
Positive IP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBG1 Ready-To-Use IHC Kit. HBG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25728-1-AP targets HBG1 in WB, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 HBG1 fusion protein Ag22776 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, gamma A
别名 Gamma 1 globin, Hb F Agamma, HBG1, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979
计算分子量 147 aa, 16 kDa
观测分子量 14-16 kDa
GenBank蛋白编号BC010913
基因名称 HBG1
Gene ID (NCBI) 3047
RRIDAB_2880212
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP69891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HBG1 antibody 25728-1-APDownload protocol
IP protocol for HBG1 antibody 25728-1-APDownload protocol
FC protocol for HBG1 antibody 25728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Adv Res

Mini and enhanced CRISPR activators for cancer therapies

Authors - Meiyu Huang
View Article
humanWB

Stem Cell Reports

FAM122A Inhibits Erythroid Differentiation through GATA1.

Authors - Jing Chen
View Article
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article