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HBG1 Polyclonal antibody

HBG1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25728-1-AP

Print datasheet

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

验证数据展示

  • WB analysis using 25728-1-AP

    WB analysis using 25728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25728-1-AP (HBG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 25728-1-AP

    IP experiment of K-562 using 25728-1-AP

    IP result of anti-HBG1 (IP:25728-1-AP, 3ug; Detection:25728-1-AP 1:300) with K-562 cells lysate 2000ug.

经过测试的应用

Positive WB detected inK-562 cells, human placenta tissue
Positive IP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBG1 Ready-To-Use IHC Kit. HBG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25728-1-AP targets HBG1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen HBG1 fusion protein Ag22776 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hemoglobin, gamma A
Synonyms Gamma 1 globin, Hb F Agamma, HBG1, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC010913
Gene Symbol HBG1
Gene ID (NCBI) 3047
RRIDAB_2880212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP69891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HBG1 antibody 25728-1-APDownload protocol
IP protocol for HBG1 antibody 25728-1-APDownload protocol
FC protocol for HBG1 antibody 25728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Reports

FAM122A Inhibits Erythroid Differentiation through GATA1.

Authors - Jing Chen
View Article
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article