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验证数据展示

  • WB analysis of recombinant proein using 19993-1-AP

    WB analysis of recombinant proein using 19993-1-AP

    WB results of 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:2000.

  • WB analysis of human placenta using 19993-1-AP

    WB analysis of human placenta using 19993-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 19993-1-AP

    FC experiment of K-562 using 19993-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human HBG1/2-Specific (19993-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HBG1/2-Specific Polyclonal antibody

HBG1/2-Specific Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 19993-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

HBG1, HBGR, HBGA, Hb F Agamma, Gamma-1-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrecombinant proein, human placenta tissue
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBG1 Ready-To-Use IHC Kit. HBG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19993-1-AP targets HBG1/2-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, gamma A
别名 HBG1, HBGR, HBGA, Hb F Agamma, Gamma-1-globin
计算分子量 16 kDa
GenBank蛋白编号NM_000559
基因名称 HBG1
Gene ID (NCBI) 3047
RRIDAB_10949629
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP69891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

实验方案

Product Specific Protocols
WB protocol for HBG1/2-Specific antibody 19993-1-APDownload protocol
FC protocol for HBG1/2-Specific antibody 19993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols