验证数据展示
经过测试的应用
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:200-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66168-1-Ig targets HBG1/2-Specific in IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | hemoglobin, gamma A |
Synonyms | Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979 |
Calculated Molecular Weight | 16 kDa |
GenBank Accession Number | NM_000559 |
Gene Symbol | HBG1 |
Gene ID (NCBI) | 3047 |
RRID | AB_2881564 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P69891 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.
实验方案
Product Specific Protocols | |
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IHC protocol for HBG1/2-Specific antibody 66168-1-Ig | Download protocol |
FC protocol for HBG1/2-Specific antibody 66168-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |