验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, SGC-7901 cells |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11746-1-AP targets HBP1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | HBP1 fusion protein Ag2342 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HMG-box transcription factor 1 |
| 别名 | HBP1, HMG box containing protein 1, HMG box transcription factor 1 |
| 计算分子量 | 514 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC022329 |
| 基因名称 | HBP1 |
| Gene ID (NCBI) | 26959 |
| RRID | AB_2247970 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60381 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMG-box protein 1 (HBP1) is a transcriptional repressor, and has a role in cell cycle progression and tumor suppression. Also it's a repressor of the cyclin D1 gene and inhibits the Wnt signaling pathway. Interaction with RB1 enhances it's binding to the H1F0 promoter . Disrupts the interaction between DNA and TCF4. This is a rabbit polyclonal antibody raised against part of C-terminus of HBP1 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HBP1 antibody 11746-1-AP | Download protocol |
| IP protocol for HBP1 antibody 11746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state. | ||
Oncogene miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.
| ||
Oncogene CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer. | ||
EMBO Rep The human GID complex engages two independent modules for substrate recruitment. | ||
J Cell Physiol Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.
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