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验证数据展示

  • WB analysis of K-562 using 83576-4-RR

    WB analysis of K-562 using 83576-4-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83576-4-RR

    Affinity and Kinetic Characterization of 83576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

HBQ1 Recombinant antibody

HBQ1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83576-4-RR

产品说明书

CloneNo. 240527D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1, Hemoglobin subunit theta 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83576-4-RR targets HBQ1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HBQ1 fusion protein Ag10105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hemoglobin, theta 1
别名 hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1, Hemoglobin subunit theta 1
计算分子量 142 aa, 16 kDa
观测分子量10~16 kDa
GenBank蛋白编号BC056686
基因名称 HBQ1
Gene ID (NCBI) 3049
RRIDAB_3671187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

实验方案

Product Specific Protocols
WB protocol for HBQ1 antibody 83576-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols