验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
15118-1-AP targets HCCS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | HCCS fusion protein Ag7189 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | holocytochrome c synthase (cytochrome c heme-lyase) |
| 别名 | Holocytochrome c-type synthase, Holocytochrome c type synthase, EC:4.4.1.17, Cytochrome c-type heme lyase, Cytochrome c type heme lyase |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC001691 |
| 基因名称 | HCCS |
| Gene ID (NCBI) | 3052 |
| RRID | AB_2114848 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53701 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HCCS antibody 15118-1-AP | Download protocol |
| IHC protocol for HCCS antibody 15118-1-AP | Download protocol |
| IF protocol for HCCS antibody 15118-1-AP | Download protocol |
| IP protocol for HCCS antibody 15118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. | ||
Chem Pharm Bull (Tokyo) The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo. | ||
medRxiv Biallelic variants in COX18 cause a mitochondrial disorder primarily manifesting as peripheral neuropathy | ||
Mol Cell Reactive oxygen species control protein degradation at the mitochondrial import gate | ||
Nat Cell Biol Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space |
