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HCFC1 Polyclonal antibody

HCFC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28569-1-AP

Print datasheet

Synonyms

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

验证数据展示

  • WB analysis using 28569-1-AP

    WB analysis using 28569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28569-1-AP (HCFC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 28569-1-AP

    IHC staining of mouse skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HCFC1 antibody included
  • IHC staining of mouse skin using 28569-1-AP

    IHC staining of mouse skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HCFC1 antibody included
  • IF Staining of HeLa using 28569-1-AP

    IF Staining of HeLa using 28569-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody (28569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of HeLa using 28569-1-AP

    IP experiment of HeLa using 28569-1-AP

    IP result of anti-HCFC1 (IP:28569-1-AP, 4ug; Detection:28569-1-AP 1:20000) with HeLa cells lysate 1600 ug.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells, C2C12 cells, C6 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skin tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat lung tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HCFC1 Ready-To-Use IHC Kit. HCFC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28569-1-AP targets HCFC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HCFC1 fusion protein Ag29663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name host cell factor C1 (VP16-accessory protein)
Synonyms C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein
Calculated Molecular Weight 209 kDa
Observed Molecular Weight300 kDa, 100-150 kDa
GenBank Accession NumberNM_005334
Gene Symbol HCFC1
Gene ID (NCBI) 3054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HCFC1 (also known as HCF-1) was discovered in studies of herpes simplex virus (HSV) transcription. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in the control of the cell cycle and transcriptional regulation during herpes simplex virus infection. HCFC1 is a large protein of ∼300 kDa and is proteolytically processed into N- and C-terminal fragments of ∼150 kDa each.(PMID: 21295698 )

实验方案

Product Specific Protocols
WB protocol for HCFC1 antibody 28569-1-APDownload protocol
IHC protocol for HCFC1 antibody 28569-1-APDownload protocol
IF protocol for HCFC1 antibody 28569-1-APDownload protocol
IP protocol for HCFC1 antibody 28569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols