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HCLS1 Polyclonal antibody

HCLS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25003-1-AP

Print datasheet

Synonyms

p75, LckBP1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1

验证数据展示

  • WB analysis of Jurkat using 25003-1-AP

    WB analysis of Jurkat using 25003-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25003-1-AP (HCLS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 25003-1-AP

    IHC staining of human tonsillitis using 25003-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25003-1-AP (HCLS1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human tonsillitis using 25003-1-AP

    IHC staining of human tonsillitis using 25003-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25003-1-AP (HCLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25003-1-AP targets HCLS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, canine
Immunogen HCLS1 fusion protein Ag8645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hematopoietic cell-specific Lyn substrate 1
Synonyms p75, LckBP1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1
Calculated Molecular Weight 486 aa, 54 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC016758
Gene Symbol HCLS1
Gene ID (NCBI) 3059
RRIDAB_2879842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14317
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.

实验方案

Product Specific Protocols
WB protocol for HCLS1 antibody 25003-1-APDownload protocol
IHC protocol for HCLS1 antibody 25003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.

Authors - Jinguo Zhang
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canineWB

Vaccines (Basel)

Transcriptional Analysis of lncRNA and Target Genes Induced by Influenza A Virus Infection in MDCK Cells

Authors - Geng Liu
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