HCN2 Polyclonal antibody

HCN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30946-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30946-1-AP targets HCN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HCN2 fusion protein Ag34038 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 2
Synonyms Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight60 kDa, 90 kDa, 120 kDa
GenBank Accession NumberNM_001194
Gene Symbol HCN2
Gene ID (NCBI) 610
RRIDAB_3086427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL51
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)

实验方案

Product Specific Protocols
WB protocol for HCN2 antibody 30946-1-APDownload protocol
IHC protocol for HCN2 antibody 30946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

CNS Neurosci Ther

HCN channels in the lateral habenula regulate pain and comorbid depressive-like behaviors in mice

Authors - Xue-Zhong Cao
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