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验证数据展示

  • WB analysis of C6 using 82921-1-RR

    WB analysis of C6 using 82921-1-RR

    C6 cells were subjected to SDS PAGE followed by western blot with 82921-1-RR (HCRTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82921-1-RR

    Affinity and Kinetic Characterization of 82921-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82921-1-RR against Human HCRTR1 were performed. The affinity constant is below 1 pM.

HCRTR1 Recombinant antibody

HCRTR1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82921-1-RR

产品说明书

CloneNo. 1E18

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

Orexin receptor 1, Ox 1 R, Orexin/Hypocretin receptor type 1, Orexin receptor type 1, Hypocretin receptor type 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82921-1-RR targets HCRTR1 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 HCRTR1 fusion protein Ag12269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypocretin (orexin) receptor 1
别名 Orexin receptor 1, Ox 1 R, Orexin/Hypocretin receptor type 1, Orexin receptor type 1, Hypocretin receptor type 1
计算分子量 425 aa, 48 kDa
观测分子量54 kDa
GenBank蛋白编号BC074796
基因名称 HCRTR1
Gene ID (NCBI) 3061
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43613
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

实验方案

Product Specific Protocols
WB protocol for HCRTR1 antibody 82921-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols