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CoraLite® Plus 647-conjugated HDAC1 Polyclonal antibody
HDAC1 Polyclonal Antibody for
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
产品信息
CL647-10197 targets HDAC1 in applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | HDAC1 fusion protein Ag0256 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histone deacetylase 1 |
| Synonyms | RPD3, histone deacetylase 1, HD1, GON 10, EC:3.5.1.98 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC000301 |
| Gene Symbol | HDAC1 |
| Gene ID (NCBI) | 3065 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13547 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HDAC1 poly https://www.ptglab.com/products/HDAC1-Antibody-10197-1-AP.htm
Background
Histone Deacetylase 1 (HDAC1) is a nuclear localized class I histone deacetylase that plays a role in the regulation of gene expression, mainly by repression of gene activity. Additionally, HDAC can mediate deacetylation of a subset of non-histone proteins, leading to their degradation. HDAC1 activity has an impact on cell growth, proliferation, and death.
1. What is the molecular weight of HDAC1?
The molecular size of HDAC1 is 60 kDa.
2. What is the subcellular localization of HDAC1?
Unlike some HDACs, HDAC1 is present entirely in the nucleus. Our HDAC1 antibody has been broadly tested for IF/ICC.
3. I cannot detect an HDAC1 specific signal in my sample during western blotting.
Make sure that you efficiently extract nuclear proteins during your sample preparation. Some lysis buffers (e.g., based on Triton X-100) may not extract nuclear proteins. We recommend using RIPA buffer (https://www.ptglab.com/support/protocols/). We highly recommend using nuclear loading control antibodies, such as lamin B1 or PCNA (https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/). Alternatively, you may consider performing cell fractionation.
4. How do I perform chromatin immunoprecipitation (ChiP) with the HDAC1 antibody?
We recommend using our standard chromatin immunoprecipitation protocol (https://www.ptglab.com/media/2708/web_chip-protocol.pdf).
5. Is HDAC1 post-translationally modified?
HDAC1 is a protein deacetylase but itself can also be a subject of post-translational modifications, including phosphorylation, acetylation, ubiquitination, SUMOylation, nitrosylation, and carbonylation (PMID: 21197454).
6. What is the role of HDAC1 in cancer?
The acetylation and deacetylation of histones play an important part in the epigenetic regulation of gene expression. Alterations in the balance between these two opposing processes changes chromosome remodeling and increased deacetylation leads to gene silencing. HDAC1 levels are increased in many cancer cell types and the role of HDAC1 in cancer is connected not only to histone deacetylation but also to the deacetylation of other proteins, such as Rb family proteins, estrogen receptors, and p53 protein (PMID: 19383284).