验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | MCF-7 cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy HDAC10 Ready-To-Use IHC Kit. HDAC10 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67646-1-Ig targets HDAC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | HDAC10 fusion protein Ag18470 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histone deacetylase 10 |
| 别名 | Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48 |
| 计算分子量 | 669 aa, 71 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC125083 |
| 基因名称 | HDAC10 |
| Gene ID (NCBI) | 83933 |
| RRID | AB_2882846 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q969S8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDAC10 antibody 67646-1-Ig | Download protocol |
| IF protocol for HDAC10 antibody 67646-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |