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HDAC10 Monoclonal antibody

HDAC10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B11

Cat No : 67646-1-Ig

Print datasheet

Synonyms

DKFZp761B039, HD10, HDAC, HDAC10, histone deacetylase 10

验证数据展示

  • WB analysis using 67646-1-Ig

    WB analysis using 67646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67646-1-Ig

    WB analysis using 67646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC10 Ready-To-Use IHC Kit. HDAC10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67646-1-Ig targets HDAC10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HDAC10 fusion protein Ag18470 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone deacetylase 10
Synonyms DKFZp761B039, HD10, HDAC, HDAC10, histone deacetylase 10
Calculated Molecular Weight 669 aa, 71 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC125083
Gene Symbol HDAC10
Gene ID (NCBI) 83933
RRIDAB_2882846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969S8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

实验方案

Product Specific Protocols
WB protocol for HDAC10 antibody 67646-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols