验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, SKOV-3 cells, T-47D cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HDAC11 Ready-To-Use IHC Kit. HDAC11 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
67949-1-Ig targets HDAC11 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | HDAC11 fusion protein Ag28335 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histone deacetylase 11 |
| 别名 | FLJ22237, HD11, HDAC11, histone deacetylase 11 |
| 计算分子量 | 347 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC009676 |
| 基因名称 | HDAC11 |
| Gene ID (NCBI) | 79885 |
| RRID | AB_2918701 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96DB2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDAC11 antibody 67949-1-Ig | Download protocol |
| IHC protocol for HDAC11 antibody 67949-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Lung Cancer Res Clinicopathological characteristics and prognostic significance of HDAC11 protein expression in non-small cell lung cancer: a retrospective study. | ||
Cell Discov Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance | ||
J South Med Univ [Overexpression of histone deacetylase 11 suppresses basal-like breast cancer cell invasion and metastasis]
| ||
