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验证数据展示

  • WB analysis of HeLa using 16164-1-AP

    WB analysis of HeLa using 16164-1-AP

    WB result of HDAC3-specific antibody (16164-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3-specific transfected HeLa cells.

  • WB analysis using 16164-1-AP

    WB analysis using 16164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

HDAC3-specific Polyclonal antibody

HDAC3-specific Polyclonal Antibody for WB, ELISA
Cat No. 16164-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HD3, HDAC3, HDAC3-specific, histone deacetylase 3, RPD3, RPD3 2, SMAP45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, human stomach tissue, HeLa cells, HL-60 cells, MOLT-4 cells, NCI-H1299 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16164-1-AP targets HDAC3-specific in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 3
别名 HD3, HDAC3, HDAC3-specific, histone deacetylase 3, RPD3, RPD3 2, SMAP45
计算分子量 49 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_003883
基因名称 HDAC3
Gene ID (NCBI) 8841
RRIDAB_2118384
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.


实验方案

Product Specific Protocols
WB protocol for HDAC3-specific antibody 16164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols