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HDAC4 Polyclonal antibody

HDAC4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29252-1-AP

Print datasheet

Synonyms

HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

验证数据展示

  • WB analysis using 29252-1-AP

    WB analysis using 29252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29252-1-AP (HDAC4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29252-1-AP

    IHC staining of human lung cancer using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 29252-1-AP

    IHC staining of human lung cancer using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 29252-1-AP

    IHC staining of human ovary tumor using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 29252-1-AP

    IHC staining of human ovary tumor using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29252-1-AP targets HDAC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen HDAC4 fusion protein Ag30752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 4
Synonyms HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288
Calculated Molecular Weight 972 aa, 106 kDa
Observed Molecular Weight120-140 kDa
GenBank Accession NumberBC039904
Gene Symbol HDAC4
Gene ID (NCBI) 9759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC4 (Histone deacetylase 4) belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylation gives a tag for epigenetic repression and plays a role in transcriptional regulation, cell cycle progression and developmental events. It is involved in muscle maturation by its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. HDAC4 is required for TGFbeta1-induced myofibroblastic differentiation (PMID: 17610967). HDAC4 encoded by an allele associated with mental retardation is a gain-of-function nuclear repressor that abolishes transcription and synaptic transmission (PMID: 23141539).

实验方案

Product Specific Protocols
WB protocol for HDAC4 antibody 29252-1-APDownload protocol
IHC protocol for HDAC4 antibody 29252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols