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验证数据展示

  • WB analysis using 29252-1-AP

    WB analysis using 29252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29252-1-AP (HDAC4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29252-1-AP

    IHC staining of human lung cancer using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC4 antibody included
  • IHC staining of human lung cancer using 29252-1-AP

    IHC staining of human lung cancer using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC4 antibody included
  • IHC staining of human ovary tumor using 29252-1-AP

    IHC staining of human ovary tumor using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC4 antibody included
  • IHC staining of human ovary tumor using 29252-1-AP

    IHC staining of human ovary tumor using 29252-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC4 antibody included

HDAC4 Polyclonal antibody

HDAC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29252-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HDAC4 Ready-To-Use IHC Kit. HDAC4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29252-1-AP targets HDAC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 HDAC4 fusion protein Ag30752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 4
别名 HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288
计算分子量 972 aa, 106 kDa
观测分子量120-140 kDa
GenBank蛋白编号BC039904
基因名称 HDAC4
Gene ID (NCBI) 9759
RRIDAB_3086107
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56524
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC4 (Histone deacetylase 4) belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylation gives a tag for epigenetic repression and plays a role in transcriptional regulation, cell cycle progression and developmental events. It is involved in muscle maturation by its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. HDAC4 is required for TGFbeta1-induced myofibroblastic differentiation (PMID: 17610967). HDAC4 encoded by an allele associated with mental retardation is a gain-of-function nuclear repressor that abolishes transcription and synaptic transmission (PMID: 23141539).

实验方案

Product Specific Protocols
WB protocol for HDAC4 antibody 29252-1-APDownload protocol
IHC protocol for HDAC4 antibody 29252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols