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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29342-1-AP (same clone as 29342-1-PBS)

    WB analysis using 29342-1-AP (same clone as 29342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29342-1-AP (HDAC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 29342-1-AP (same clone as 29342-1-PBS)

    IHC staining of mouse brain using 29342-1-AP (same clone as 29342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29342-1-AP (HDAC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC5 antibody included
  • IF Staining of HepG2 using 29342-1-AP (same clone as 29342-1-PBS)

    IF Staining of HepG2 using 29342-1-AP (same clone as 29342-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5 antibody (29342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

HDAC5 Polyclonal antibody, PBS Only

HDAC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29342-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HDAC5 Ready-To-Use IHC Kit. HDAC5 primary antibody included.

产品信息

29342-1-PBS targets HDAC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HDAC5 fusion protein Ag29122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 5
别名
计算分子量 122 kDa
观测分子量120-140 kDa
GenBank蛋白编号NM_005474
基因名称 HDAC5
Gene ID (NCBI) 10014
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQL6
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.