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Species Reactivity
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Species Reactivity
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验证数据展示

  • IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

    IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC5 antibody included
  • WB analysis of HeLa using 16166-1-AP (same clone as 16166-1-PBS)

    WB analysis of HeLa using 16166-1-AP (same clone as 16166-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16166-1-AP (same clone as 16166-1-PBS)

    IHC staining of human brain using 16166-1-AP (same clone as 16166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC5 antibody included
  • WB analysis using 16166-1-AP (same clone as 16166-1-PBS)

    WB analysis using 16166-1-AP (same clone as 16166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16166-1-AP (same clone as 16166-1-PBS)

    IF Staining of HepG2 using 16166-1-AP (same clone as 16166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5-specific antibody (16166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HDAC5-specific Polyclonal antibody, PBS Only

HDAC5-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16166-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HDAC5 Ready-To-Use IHC Kit. HDAC5 primary antibody included.

产品信息

16166-1-PBS targets HDAC5-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 5
Synonyms
Calculated Molecular Weight 122 kDa
Observed Molecular Weight120-140 kDa
GenBank Accession NumberBC051824
Gene Symbol HDAC5
Gene ID (NCBI) 10014
RRIDAB_2116779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQL6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.