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验证数据展示

  • IHC staining of human testis using 23328-1-AP

    IHC staining of human testis using 23328-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23328-1-AP (HDAC9 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SH-SY5Y using 23328-1-AP

    IF Staining of SH-SY5Y using 23328-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC9 antibody (23328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HDAC9 Polyclonal antibody

HDAC9 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 23328-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

HD9, EC:3.5.1.98, Histone deacetylase 9, HDRP, HDAC7B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23328-1-AP targets HDAC9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HDAC9 fusion protein Ag19930 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 9
别名 HD9, EC:3.5.1.98, Histone deacetylase 9, HDRP, HDAC7B
计算分子量 1011 aa, 111 kDa
观测分子量 100 kDa
GenBank蛋白编号BC152405
基因名称 HDAC9
Gene ID (NCBI) 9734
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9UKV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HDAC9 antibody 23328-1-APDownload protocol
IF protocol for HDAC9 antibody 23328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols