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验证数据展示

  • WB analysis using 28334-1-AP

    WB analysis using 28334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28334-1-AP (HDAC9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 28334-1-AP

    IF Staining of SH-SY5Y using 28334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC9 antibody (28334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HDAC9 Polyclonal antibody

HDAC9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28334-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

DKFZp779K1053, HD7, HD7b, HD9, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, MDA-MB-231 cells, MDA-MB-468 cells, mouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28334-1-AP targets HDAC9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 HDAC9 fusion protein Ag28514 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 9
别名 DKFZp779K1053, HD7, HD7b, HD9, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR
计算分子量 1011 aa, 111 kDa
观测分子量57 kDa
GenBank蛋白编号BC152405
基因名称 HDAC9
Gene ID (NCBI) 9734
RRIDAB_3669652
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle, and testis. HDAC9 has 11 isoforms produced by alternative splicing. The observed molecular weight of HDAC9 is 57 kDa, which represents alternate isoforms of HDAC9.

实验方案

Product Specific Protocols
WB protocol for HDAC9 antibody 28334-1-APDownload protocol
IF protocol for HDAC9 antibody 28334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols