验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Daudi cells, HepG2 cells, Raji cells, K-562 cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67364-1-Ig targets HDAC9 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | HDAC9 fusion protein Ag28514 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histone deacetylase 9 |
| 别名 | DKFZp779K1053, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR |
| 计算分子量 | 1011 aa, 111 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC152405 |
| 基因名称 | HDAC9 |
| Gene ID (NCBI) | 9734 |
| RRID | AB_2882616 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UKV0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle and testis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDAC9 antibody 67364-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Ophthalmol Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism | ||
Cell Death Dis WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway. |
