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CoraLite® Plus 488-conjugated HDDC2 Monoclonal antibody

HDDC2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E1B2

Cat no : CL488-68391

Print datasheet

Synonyms

EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2

验证数据展示

  • IF Staining of A431 using CL488-68391

    IF Staining of A431 using CL488-68391

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 HDDC2 antibody (CL488-68391, Clone: 2E1B2 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of U2OS using CL488-68391

    FC experiment of U2OS using CL488-68391

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HDDC2 Monoclonal antibody (CL488-68391, Clone:2E1B2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68391-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68391 targets HDDC2 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen HDDC2 fusion protein Ag33258 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name HD domain containing 2
Synonyms EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC003357
Gene Symbol HDDC2
Gene ID (NCBI) 51020
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z4H3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HDDC2 antibody CL488-68391Download protocol
Standard Protocols
Click here to view our Standard Protocols