HDGF Monoclonal antibody
HDGF Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
5A7G3
验证数据展示
经过测试的应用
| Positive WB detected in | SMMC-7721 cells, A549 cells, HeLa cells, HepG2 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HDGF Ready-To-Use IHC Kit. HDGF primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60064-1-Ig targets HDGF in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | HDGF fusion protein Ag1893 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | hepatoma-derived growth factor (high-mobility group protein 1-like) |
| Synonyms | HDGF, Hepatoma derived growth factor, HMG 1L2, HMG1L2 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC018991 |
| Gene Symbol | HDGF |
| Gene ID (NCBI) | 3068 |
| RRID | AB_2117098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P51858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDGF antibody 60064-1-Ig | Download protocol |
| IHC protocol for HDGF antibody 60064-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. | ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma. | ||
Neural Plast Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy. | ||
Biochem Biophys Res Commun Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma. | ||
Med Oncol The expression of hepatoma-derived growth factor in primary central nervous system lymphoma and its correlation with angiogenesis, proliferation and clinical outcome. | ||
Mol Vis The regulatory role of hepatoma-derived growth factor as an angiogenic factor in the eye. |
