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  • KD/KO Validated

HDGF2 Polyclonal antibody

HDGF2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 15134-1-AP

Print datasheet

Synonyms

HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2



经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy HDGFL2 Ready-To-Use IHC Kit. HDGFL2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15134-1-AP targets HDGF2 in IHC, IF/ICC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF, CoIP, chIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen HDGF2 fusion protein Ag7281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hepatoma-derived growth factor-related protein 2
Synonyms HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2
Calculated Molecular Weight 74 kDa
GenBank Accession NumberBC000755
Gene Symbol HDGF2
Gene ID (NCBI) 84717
RRIDAB_2117220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4V5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.

实验方案

Product Specific Protocols
IHC protocol for HDGF2 antibody 15134-1-APDownload protocol
IF protocol for HDGF2 antibody 15134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Sci Transl Med

Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD

Authors - Sahba Seddighi
humanWB

Sci Adv

The H3K36me2 writer-reader dependency in H3K27M-DIPG.

Authors - Jia-Ray Yu
  • KD Validated
humanWB

Sci Adv

LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.

Authors - Gary LeRoy
mouseWB,chIP

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu
  • KD Validated
humanIF, IP

Nucleic Acids Res

Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.

Authors - Annika Baude
humanWB

Cell Rep

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.

Authors - Judd F Hultquist
  • KO Validated