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  • Featured Product
  • KD/KO Validated

HDLBP Polyclonal antibody

HDLBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15406-1-AP

Print datasheet

Synonyms

Vigilin, VGL, PRO2900, High density lipoprotein-binding protein, HDL-binding protein

验证数据展示

  • WB analysis using 15406-1-AP

    WB analysis using 15406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15406-1-AP (HDLBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 15406-1-AP

    IF Staining of MCF-7 using 15406-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HDLBP antibody 15406-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of Jurkat using 15406-1-AP

    IP experiment of Jurkat using 15406-1-AP

    IP result of anti-HDLBP (IP:15406-1-AP, 4ug; Detection:15406-1-AP 1:500) with Jurkat cells lysate 2400ug.

  • IHC staining of mouse stomach using 15406-1-AP

    IHC staining of mouse stomach using 15406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 15406-1-AP

    IHC staining of mouse stomach using 15406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15406-1-AP targets HDLBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HDLBP fusion protein Ag7666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high density lipoprotein binding protein
Synonyms Vigilin, VGL, PRO2900, High density lipoprotein-binding protein, HDL-binding protein
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 141 kDa
GenBank Accession NumberBC001179
Gene Symbol HDLBP
Gene ID (NCBI) 3069
RRIDAB_2117367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00341
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HDLBP antibody 15406-1-APDownload protocol
IHC protocol for HDLBP antibody 15406-1-APDownload protocol
IF protocol for HDLBP antibody 15406-1-APDownload protocol
IP protocol for HDLBP antibody 15406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Cell Res

A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.

Authors - Wei Zhou
  • KD Validated
View Article
humanWB

J Med Virol

Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis.

Authors - Zhao Chao C
View Article
humanWB,IHC,IF,CoIP

Cell Mol Gastroenterol Hepatol

HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation

Authors - Jingsheng Yuan
View Article