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验证数据展示

  • WB analysis using 24578-1-AP

    WB analysis using 24578-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24578-1-AP (HEATR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 24578-1-AP

    IHC staining of human stomach using 24578-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24578-1-AP (HEATR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 24578-1-AP

    IF Staining of HEK-293 using 24578-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HEATR2 antibody (24578-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

HEATR2 Polyclonal antibody

HEATR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24578-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

HEAT repeat-containing protein 2, HEAT repeat containing 2, Dynein axonemal assembly factor 5, DNAAF5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24578-1-AP targets HEATR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, rat
免疫原 HEATR2 fusion protein Ag20080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HEAT repeat containing 2
别名 HEAT repeat-containing protein 2, HEAT repeat containing 2, Dynein axonemal assembly factor 5, DNAAF5
计算分子量 855 aa, 94 kDa
观测分子量 93 kDa
GenBank蛋白编号BC047240
基因名称 HEATR2
Gene ID (NCBI) 54919
RRIDAB_2827668
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86Y56
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEATR2 also termed as HEAT repeat containing 2 is an 855 amino acid protein. HEATR2 is a member of a family of ten other uncharacterized HEAT-repeat-containing proteins, which contains HEAT (Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A) and the yeast PI3-kinase Tor1) repeats. HEAT repeats form rod-like helical structures that are involved in intracellular transport. HEATR2 play an important role in dynein arm transportation or assembly. HEATR2 mutation is implicated in Primary ciliary dyskinesia 18.

实验方案

Product Specific Protocols
WB protocol for HEATR2 antibody 24578-1-APDownload protocol
IHC protocol for HEATR2 antibody 24578-1-APDownload protocol
IF protocol for HEATR2 antibody 24578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

DNA Methylation Regulates Alternative Polyadenylation via CTCF and the Cohesin Complex.

Authors - Vishal Nanavaty
View Article
rat,humanWB, IF

PLoS Genet

HEATR2 Plays a Conserved Role in Assembly of the Ciliary Motile Apparatus.

Authors - Christine P Diggle
View Article
humanWB

Front Oncol

DNAAF5 promotes hepatocellular carcinoma malignant progression by recruiting USP39 to improve PFKL protein stability

Authors - Yaping Liu
View Article