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HEATR3 Polyclonal antibody

HEATR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27335-1-AP

Print datasheet

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3

验证数据展示

  • WB analysis using 27335-1-AP

    WB analysis using 27335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27335-1-AP (HEATR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27335-1-AP

    IHC staining of human ovary cancer using 27335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27335-1-AP (HEATR3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27335-1-AP targets HEATR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen HEATR3 fusion protein Ag26328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HEAT repeat containing 3
Synonyms HEAT repeat-containing protein 3, HEAT repeat containing 3
Calculated Molecular Weight 680 aa, 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC033077
Gene Symbol HEATR3
Gene ID (NCBI) 55027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4Q2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HEATR3 antibody 27335-1-APDownload protocol
IHC protocol for HEATR3 antibody 27335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols