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验证数据展示

  • WB analysis using 16842-1-AP

    WB analysis using 16842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16842-1-AP (HEBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 16842-1-AP

    WB analysis of A549 using 16842-1-AP

    WB result of HEBP1 antibody (16842-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • IHC staining of human liver cancer using 16842-1-AP

    IHC staining of human liver cancer using 16842-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

HEBP1 Polyclonal antibody

HEBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16842-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

p22HBP, Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, A549 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16842-1-AP targets HEBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HEBP1 fusion protein Ag10538 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heme binding protein 1
别名 p22HBP, Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP
计算分子量 189 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC016277
基因名称 HEBP1
Gene ID (NCBI) 50865
RRIDAB_2117378
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEBP1 (heme binding protein 1) is an intracellular tetrapyrrole-binding protein. Catalog#16842-1-AP is a rabbit polyclonal antibody raised against the full-length of human HEBP1. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein.

实验方案

Product Specific Protocols
WB protocol for HEBP1 antibody 16842-1-APDownload protocol
IHC protocol for HEBP1 antibody 16842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols